This antibody recognises bovine Alpha-2-HS-glycoprotein, also known as asialofetuin or fetuin-A. Asialofetuin is a 359 amino acid ~55kDa secreted glycoprotein containing two fetuin-A-type dystatin domains.Asialofetuin is produced in the liver and bone marrow and acts as a negative acute-phase protein attenuating the inflammatory response to acute injury. In a simple antigen down ELISA, Sheep anti Bovine asialofetuin antibody reacts with:asialofetuin (ab at 1/8000) 100% fetuin (ab at 1/2000) 100% fetuin (ab at 1/8000) 50%
Applications
ELISA
Dilutions
ELISA: 1:1,000 - 1:4,000
Reactivity
Bovine
Immunogen
Native Asialofetuin protein.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein G affinity chromatography.
Concentration
5 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Bovine asialofetuin antibody recognizes bovine Alpha-2-HS-glycoprotein, also known as asialofetuin or fetuin-A. Asialofetuin is a 359 amino acid ~55kDa secreted glycoprotein containing two fetuin-A-type dystatin domains.Asialofetuin is produced in the liver and bone marrow and acts as a negative acute-phase protein attenuating the inflammatory response to acute injury (Wang et al. 2010).In a simple antigen down ELISA, Sheep anti Bovine asialofetuin antibody reacts with:asialofetuin (ab at 1/8000) 100%fetuin (ab at 1/2000) 100%fetuin (ab at 1/8000) 50%
