ASIC1 expression in Mouse fetal Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ASIC1 Antibody (A83063) at 0.3µg/ml and detected by chemiluminescence.
ASIC1 expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ASIC1 Antibody (A83063) at 5µg/ml and revealed with alkaline phosphatase (AP).
ASIC1 expression in Human Pancreas analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ASIC1 Antibody (A83063) at 5µg/ml and revealed with alkaline phosphatase (AP).
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