This antibody recognises cyclic AMP-dependent transcription factor ATF1, also known as activating transcription factor 1 or protein TREB36.ATF1 is a protein belonging to the ATF subfamily of the bZIP (basic-region leucine zipper) family involved in the regulation of the expression of genes involved in several cellular processes including cell growth and survival. The ATF1 protein is phosphorylated at serine 63 in its kinase-inducible domain by several serine/threonine kinases, including cAMP-dependent protein kinase A and cyclin-dependent kinase 3 (CDK3). Phosphorylation of ATF1 at serine 63 enhances its transactivation and transcriptional activity, thereby enhancing cell transformation.
Applications
WB
Dilutions
WB: 1:500 - 1:1,000
Reactivity
Human
Immunogen
A peptide derived from within the C-terminus of human ATF1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Human ATF1 antibody recognizes cyclic AMP-dependent transcription factor ATF1, also known as activating transcription factor 1 or protein TREB36.ATF1 is aprotein belonging to the ATF subfamily of thebZIP(basic-region leucine zipper) family involved in the regulation of the expression of genes involved in several cellular processes including cell growth and survival. The ATF1 protein is phosphorylated at serine 63 in its kinase-inducible domain by several serine/threonine kinases, including cAMP-dependent protein kinase A and cyclin-dependent kinase 3(CDK3). Phosphorylation of ATF1 at serine 63 enhances its transactivation and transcriptional activity, thereby enhancing cell transformation (Zheng et al. 2008).
