Notice: Error: The total number of locks exceeds the lock table size Error No: 1206 INSERT INTO oc_analytics set ip = '', agent = 'Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)', url = '/catalog/primary-antibodies/atg4d-antibody-a304858', route = 'product/product', port = '62345', country_code ='US', country_name='United States', city='Columbus', organisation='', referrer='', adflag='N', adcampaign='', adurl='', product_id='304858' in D:\wwwroot_antibodies\system\library\db\mysqli.php on line 42Anti-ATG4D Antibody (A304858) | Antibodies.com
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of 52.9 kDa ATG4D protein using Anti-ATG4D Antibody (A304858) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: human HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa.
Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG4D Antibody (A304858), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4D Antibody. (D) Composite.
Publishing research using Anti-ATG4D Antibody (A304858)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-ATG4D Antibody (A304858)
