Anti-ATP5F1 Antibody (A85201)

Name: Anti-ATP5F1 Antibody
Brand: Antibodies.com
SKU: A85201
Price: 115.0 GBP
Availability: InStock

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$495

Goat polyclonal antibody to ATP5F1 for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-ATP5F1 Antibody

Description

Goat polyclonal antibody to ATP5F1.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:128,000, WB: 0.03-0.1µg/ml, IHC: 5µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 142-153, of human ATP5F1.

Sequence

C-SIQHIQNAIDTE

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

25 kDa

Predicted MW

28.9kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ATP synthase F(0) complex subunit B1, mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATP5PB, ATPase subunit b

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-ATP5F1 Antibody (A85201)

ATP5F1 expression in Human Heart lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ATP5F1 Antibody (A85201) at 0.03µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-ATP5F1 Antibody (A85201)

ATP5F1 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ATP5F1 Antibody (A85201) at 5µg/ml and revealed with alkaline phosphatase (AP).

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