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Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808)

Western Blot - Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808) - Antibodies.com Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to ATPase Inhibitory Factor 1/IF1 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-ATPase Inhibitory Factor 1/IF1 Antibody
Description
Rabbit polyclonal antibody to ATPase Inhibitory Factor 1/IF1.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1).
Sequence
GSDQSENVDRGAGSIREAGGAFGKREQAEEERYFRAQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLKHDD
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
12 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808) - Antibodies.comEnlarge Image

Western Blot - Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808)

Western blot analysis of extracts from wild type(WT) and ATPIF1 knockout (KO) 293T(KO) cells, using Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808)

Immunofluorescence analysis of U2OS cells using Anti-ATPase Inhibitory Factor 1/IF1 Antibody (A11808) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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