Rabbit polyclonal antibody to b-Catenin (Phospho-Thr41 / Ser45)
Specificity
The antibody detects endogenous level of b-Catenin only when phosphorylated at threonine 41 / serine 45.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 41 / serine 45 (A-T-T(p)-T-A-P-S(p)-L-S) derived from Human b-Catenin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
92kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from SW626 cells using b-Catenin (Phospho-Thr41/Ser45) Antibody #11116 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
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