Anti-B2M Antibody (A29785)

Applications: Western Blot

Species: Human and Mouse

Sample: Human and mouse cell lines

Experimental Conditions: WB: 30 mg lysate from human and mouse cell lines prepared fresh in RIPA buffer [10 mM Tris-Cl (pH 8.0), 1 mM EDTA, 140 mM NaCl, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS] with protease inhibitor cocktail (1:50, Thermo Scientific, A32963); native PAGE, 10%; PVDF membrane; tank transfer at 120-180 V for 60 min; blocked in 5% non-fat milk in TBS-T for 1 h; primary antibody overnight at 4C; washed and incubated with secondary antibody.

Dilution: Primary: 1:500

Results Summary: Successful detection of B2M via western blot in human and mouse cell lines (DT, CRH, MIAPACA, A8184, and PANC1) using Anti-B2M Antibody (A29785).