Rabbit polyclonal antibody to BAD (Phospho-Ser91 / 128)
Specificity
The antibody detects endogenous levels of BAD only when phosphorylated at serine 91 / serine 128.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 91 (E-P-S(p)-P-F) derived from Human BAD or Serine 128 (E-P-S(p)-P-F) derived from Mouse BAD.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BBC6, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-associated agonist of cell death, Bcl2-L-8, BCL2L8
Western blot analysis of extracts from COS7 cells treated with TNF-a using BAD (Phospho-Ser91/128) Antibody #11685.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser91/128) antibody #11685 (left)or the same antibody preincubated with blocking peptide (right).
Alternative products to Anti-BAD (Phospho-Ser91 / 128) Antibody (A50403)
