Anti-BAF155 Antibody [ARC0948] (A329861)

Name: Anti-BAF155 Antibody [ARC0948]
Brand: Antibodies.com
SKU: A329861
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0948] antibody to BAF155 for WB, ICC/IF, IP, ChIP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-BAF155 Antibody [ARC0948]

Description

Rabbit monoclonal [ARC0948] antibody to BAF155.

Applications

WB, ICC/IF, IP, ChIP, ELISA

Positive Control

HeLa, HepG2, Mouse brain, Mouse testis, 293T.

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration), ChIP: 5µg antibody for 10µg-15µg of Chromatin

Reactivity

Human, Mouse

Immunogen

A recombinant fusion protein corresponding to amino acids 700-800 of human BAF155.

Sequence

AFLASVVDPRVASAAAKAALEEFSRVREEVPLELVEAHVKKVQEAARASGKVDPTYGLESSCIAGTGPDEPEKLEGAEEEKMEADPDGQQPEKAENKVENE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0948

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

155 kDa

Predicted MW

123 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 0.05% BSA, 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF complex subunit SMARCC1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-BAF155 Antibody [ARC0948] (A329861)

BAF155 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-BAF155 Antibody [ARC0948] (A329861) at a dilution of 1:500 and detected with chemiluminescence.

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Western Blot - Anti-BAF155 Antibody [ARC0948] (A329861)

BAF155 expression in lysates from wild type (WT) and SMARCC1/BAF155 knockout (KO) 293T cells analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-BAF155 Antibody [ARC0948] (A329861) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunofluorescence - Anti-BAF155 Antibody [ARC0948] (A329861)

BAF155 expression in NIH/3T3 cells analyzed by immunofluorescence. Staining was performed with Anti-BAF155 Antibody [ARC0948] (A329861) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunoprecipitation - Anti-BAF155 Antibody [ARC0948] (A329861)

Chromatin immunoprecipitation (ChIP) analysis of extracts of MCF7 beta-estradiol cells, using Anti-BAF155 Antibody [ARC0948] (A329861) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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