Anti-Baf180 Antibody [ARC1820] (A309071)

Name: Anti-Baf180 Antibody [ARC1820]
Brand: Antibodies.com
SKU: A309071
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1820] antibody to Baf180 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Baf180 Antibody [ARC1820]

Description

Rabbit monoclonal [ARC1820] antibody to Baf180.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 530-680 of human PBRM1 (Q86U86).

Sequence

VLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1820

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

BRG1-associated factor 180, hPB1, PB1, Polybromo-1D, Protein polybromo-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Baf180 Antibody [ARC1820] (A309071)

Western blot analysis of extracts of various cell lines, using Anti-Baf180 Antibody [ARC1820] (A309071) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Baf180 Antibody [ARC1820] (A309071) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Baf180 Antibody [ARC1820] (A309071) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Baf180 Antibody [ARC1820] (A309071) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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