Anti-BAF57/SMARCE1 Antibody (A92974)

Name: Anti-BAF57/SMARCE1 Antibody
Brand: Antibodies.com
SKU: A92974
Price: 325.0 GBP
Availability: InStock

Enlarge Image

$485

Rabbit polyclonal antibody to BAF57/SMARCE1 for WB, IHC, ICC/IF and IP.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-BAF57/SMARCE1 Antibody

Description

Rabbit polyclonal antibody to BAF57/SMARCE1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:20-1:50

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human BAF57/BAF57/SMARCE1 (NP_003070.3).

Sequence

MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

BAF57, BRG1 associated factor 57, BRG1-associated factor 57, Chromatin remodeling complex BRG1 associated factor 57, FLJ35648, SMARCE 1, SMARCE1, SMCE1_HUMAN, SWI/SNF related matrix associated actin dependent regulator of chromatin e1, SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Enlarge Image

Western Blot - Anti-BAF57/SMARCE1 Antibody (A92974)

Western blot analysis of extracts of various cell lines, using Anti-BAF57/SMARCE1 Antibody (A92974) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Western Blot - Anti-BAF57/SMARCE1 Antibody (A92974)

Western blot analysis of extracts from normal (control) and BAF57/SMARCE1 knockout (KO) 293T cells, using Anti-BAF57/SMARCE1 Antibody (A92974) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Enlarge Image

Immunofluorescence - Anti-BAF57/SMARCE1 Antibody (A92974)

Immunofluorescence analysis of HeLa cells using Anti-BAF57/SMARCE1 Antibody (A92974).

Enlarge Image

Western Blot - Anti-BAF57/SMARCE1 Antibody (A92974)

Immunoprecipitation analysis of 150µg extracts of Jurkat cells using 3µg of Anti-BAF57/SMARCE1 Antibody (A92974). This Western blot was performed on the immunoprecipitate using Anti-BAF57/SMARCE1 Antibody (A92974) at a dilution of 1:1000.

Publishing research using Anti-BAF57/SMARCE1 Antibody (A92974)? Please let us know so that we can list the citation on this page.