Western blot analysis of extracts of THP-1 cells, using Anti-Bak Antibody (A12537) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of C6 cells using Anti-Bak Antibody (A12537) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-Bak Antibody (A12537) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 300µg extracts of THP-1 cells using 3µg of Anti-Bak Antibody (A12537). This Western blot was performed on the immunoprecipitate using Anti-Bak Antibody (A12537) at a dilution of 1:1000.
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