Anti-Bax Antibody [2D2] (CF®594) (A352100)

Anti-Bax Antibody [2D2] (CF®594)

Mouse monoclonal [2D2] antibody to Bax (CF®594).

Flow Cytometry (Intracellular), IF, IHC, IHC-P

Jurkat, K562, HL-60, or HeLa cells; Reed-Sternberg cells in Hodgkin lymphoma.

Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, WB: 0.5-1 µg/mL, IHC-P: 0.5-1 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM Tris buffer containing 1 mM EDTA, pH 9.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Human, Monkey

No cross-reactivity with Mouse or Rat. Cross-reactivity with other species has not been determined.

Synthetic peptide corresponding to amino acids 3-16 (Cys-GSGEQPRGGGPTSS) of human Bax.

Mouse

2D2

IgG1

0.1 mg/mL

21 kDa

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BCL2L4

This product is for research use only. It is not intended for diagnostic or therapeutic use.