Anti-Bcl-2 Antibody [100/D5] (Biotin) (A352160)

Anti-Bcl-2 Antibody [100/D5] (Biotin)

Mouse monoclonal [100/D5] antibody to Bcl-2 (Biotin).

Flow Cytometry (Intracellular), IHC, IHC-P, WB

Jurkat, K562, HL-60, and HeLa cells; tonsil tissue and follicular lymphoma.

Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, WB: 0.5-1 µg/mL, IHC-P: 0.5-1 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM Tris buffer containing 1 mM EDTA, pH 9.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Human

No cross-reactivity with Mouse or Rat. Cross-reactivity with other species has not been determined.

Synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2.

Mouse

Monoclonal

IgG1

kappa

0.1 mg/mL

25-26 kDa

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Apoptosis regulator Bcl-2, BCL2

This product is for research use only. It is not intended for diagnostic or therapeutic use.