Recombinant Anti-Bcl-2 Antibody [rBCL2/782] (CF®405S) (A353861)

Anti-Bcl-2 Antibody [rBCL2/782] (CF®405S)

Recombinant mouse monoclonal [rBCL2/782] antibody to Bcl-2 (CF®405S).

IHC, IHC-P, WB

Jurkat, K562, HL-60, or HeLa cells and tonsil or follicular lymphoma tissue.

WB: 0.5-1 µg/mL, IHC-P: 0.5-1 µg/mL for 30 minutes at room temperature. For IHC-P, heat-induced epitope retrieval is recommended using 1 mM EDTA buffer, pH 8.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Human

Recombinant full-length human Bcl-2 protein.

Mouse

Monoclonal

IgG1

kappa

0.1 mg/mL

25-26 kDa

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Apoptosis regulator Bcl-2, BCL2

This product is for research use only. It is not intended for diagnostic or therapeutic use.