This antibody specifically recognizes the C-terminal domain of human BDKRB2, detecting the non-phosphorylated form. It is suitable for Western blot analysis, enables immunoprecipitation from cell and tissue lysates, and is optimized for immunohistochemistry in cultured cells and tissue sections.
Applications
WB, IHC
Dilutions
WB: 1:1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human BDKRB2. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Localization of Bradykinin Receptor 2 (BR2) in pancreatic islets by immunohistochemistry. Pancreatic sections were dewaxed, microwaved in citric acid, and stained with anti-BR2 antibody (A334472) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. BR2 receptors were detected in pancreatic islet cells.
Western blot confirmation of Bradykinin Receptor 2 (BR2) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or those stably expressing BR2 were probed with the phosphorylation-independent anti-BR2 antibody (A334472) at a 1:1000 dilution.
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