Anti-Beclin 2 Antibody (A305170)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Beclin 2 Antibody (A305170), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite.

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~48.1 kDa Beclin2 protein using Anti-Beclin 2 Antibody (A305170) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: human Embryonic kidney epithelial cell line (HEK293T) lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~48.1 kDa.

Western blot analysis of rat liver showing detection of ~48kDa Beclin-2 protein using Anti-Beclin 2 Antibody (A305170) at 1:200 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat liver (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~48kDa.