Anti-beta II Tubulin Antibody [ARC0227] (A308205)

Name: Anti-beta II Tubulin Antibody [ARC0227]
Brand: Antibodies.com
SKU: A308205
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0227] antibody to beta II Tubulin for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-beta II Tubulin Antibody [ARC0227]

Description

Rabbit monoclonal [ARC0227] antibody to beta II Tubulin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 346-445 of human ßII-Tubulin/ß2-Tubulin (Q13885).

Sequence

PNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0227

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

TUBB2, TUBB2A, TUBB2B, TUBB2C, TUBB4B, Tubulin beta class IIa, Tubulin beta-2 chain, Tubulin beta-2A chain, Tubulin beta-2B chain, Tubulin beta-2C chain, Tubulin beta-4B chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-beta II Tubulin Antibody [ARC0227] (A308205)

Western blot analysis of extracts of various cell lines, using Anti-beta II Tubulin Antibody [ARC0227] (A308205) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-beta II Tubulin Antibody [ARC0227] (A308205)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-beta II Tubulin Antibody [ARC0227] (A308205) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-beta II Tubulin Antibody [ARC0227] (A308205)

Immunofluorescence analysis of C6 cells using Anti-beta II Tubulin Antibody [ARC0227] (A308205) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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