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Anti-BNIP3L/NIX Antibody (A15147)

Western Blot - Anti-BNIP3L/NIX Antibody (A15147) - Antibodies.com Immunohistochemistry - Anti-BNIP3L/NIX Antibody (A15147) - Antibodies.com Immunohistochemistry - Anti-BNIP3L/NIX Antibody (A15147) - Antibodies.com Immunofluorescence - Anti-BNIP3L/NIX Antibody (A15147) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to BNIP3L/NIX for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-BNIP3L/NIX Antibody
Description
Rabbit polyclonal antibody to BNIP3L/NIX.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 95-180 of human BNIP3L (NP_004322.1).
Sequence
SPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
45 kDa / 70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-BNIP3L/NIX Antibody (A15147) - Antibodies.comEnlarge Image

Western Blot - Anti-BNIP3L/NIX Antibody (A15147)

Western blot analysis of extracts of various cell lines, using Anti-BNIP3L/NIX Antibody (A15147) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-BNIP3L/NIX Antibody (A15147) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-BNIP3L/NIX Antibody (A15147)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-BNIP3L/NIX Antibody (A15147) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-BNIP3L/NIX Antibody (A15147) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-BNIP3L/NIX Antibody (A15147)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-BNIP3L/NIX Antibody (A15147) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-BNIP3L/NIX Antibody (A15147) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-BNIP3L/NIX Antibody (A15147)

Immunofluorescence analysis of NIH/3T3 cells using Anti-BNIP3L/NIX Antibody (A15147) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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