Anti-Bovine IgG2 Antibody [IL-A73] (A280736)

Anti-Bovine IgG2 Antibody [IL-A73]

Mouse monoclonal [IL-A73] antibody to Bovine IgG2.

This antibody recognises bovine IgG2 and does not recognise other bovine immunoglobulin classes.While normal serum levels of IgG1 are relatively constant in cattle at around 50% , IgG2 levels are highly variable between different strains and races. Mouse anti Bovine IgG2, clone IL-A73 immunoprecipitates a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2.

IP, Flow Cytometry, ELISA

ELISA: 1:20 000 - 1:200 000, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Bovine

Purified bovine IgG2.

Mouse

Monoclonal

IL-A73

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Bovine IgG2, clone IL-A73, is a monoclonal antibody specific for bovine IgG2 and does not recognise other bovine immunoglobulin classes.While normal serum levels of IgG1 are relatively constant in cattle at around 50%, IgG2 levels are highly variable between different strains and races. Mouse anti Bovine IgG2, clone IL-A73 immunoprecipitates a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2 (Campbell et al. 1998).