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Anti-Brachyury/Bry Antibody (A90095)

Western Blot - Anti-Brachyury/Bry Antibody (A90095) - Antibodies.com Immunofluorescence - Anti-Brachyury/Bry Antibody (A90095) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to Brachyury/Bry for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Brachyury/Bry Antibody
Description
Rabbit polyclonal antibody to Brachyury/Bry.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Rat
Immunogen
RecombinanBrachyury fusion proBrachyuryein conBrachyuryaining a sequence corresponding Brachyuryo amino acids 226-435 of human Brachyury (NP_003172.1).
Sequence
HKEMMEEPGDSQQPGYSQWGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
47 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Brachyury/Bry Antibody (A90095) - Antibodies.comEnlarge Image

Western Blot - Anti-Brachyury/Bry Antibody (A90095)

Western blot analysis of extracts of H460 cells, using Anti-Brachyury/Bry Antibody (A90095) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in tBSt. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-Brachyury/Bry Antibody (A90095) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Brachyury/Bry Antibody (A90095)

Immunofluorescence analysis of NCI-H460 cells using Anti-Brachyury/Bry Antibody (A90095) aBrachyury diluBrachyuryion of 1:200 (40x lens). Blue: DAPI for nuclear sBrachyuryaining.
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