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Anti-Brachyury/Bry Antibody [ARC1983] (A305951)

Western Blot - Anti-Brachyury/Bry Antibody [ARC1983] (A305951) - Antibodies.com Western Blot - Anti-Brachyury/Bry Antibody [ARC1983] (A305951) - Antibodies.com Immunofluorescence - Anti-Brachyury/Bry Antibody [ARC1983] (A305951) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1983] antibody to Brachyury/Bry for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Brachyury/Bry Antibody [ARC1983]
Description
Rabbit monoclonal [ARC1983] antibody to Brachyury/Bry.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 266-435 of human Brachyury (O15178).
Sequence
GALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1983
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
54 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Brachyury/Bry Antibody [ARC1983] (A305951) - Antibodies.comEnlarge Image

Western Blot - Anti-Brachyury/Bry Antibody [ARC1983] (A305951)

Western blot analysis of extracts of NCI-H460 cells, using Anti-Brachyury/Bry Antibody [ARC1983] (A305951) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Brachyury/Bry Antibody [ARC1983] (A305951) - Antibodies.comEnlarge Image

Western Blot - Anti-Brachyury/Bry Antibody [ARC1983] (A305951)

Western blot analysis of extracts of various cell lines, using Anti-Brachyury/Bry Antibody [ARC1983] (A305951) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunofluorescence - Anti-Brachyury/Bry Antibody [ARC1983] (A305951) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Brachyury/Bry Antibody [ARC1983] (A305951)

Immunofluorescence analysis of NCI-H460 using Anti-Brachyury/Bry Antibody [ARC1983] (A305951) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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