This antibody recognises bromodomain-containing protein 3, also known as RING3-like protein (RING3L).BRD3 is a member of the bromodomain and extra-terminal motif (BET) protein family. As with other BET proteins, BRD3 associates with acetylated lysine residues on histones and transcription factors. BRD3 is thought to be involved in nucleosome remodelling in the context of transcription. BRD3 has also been shown to interact with RNA molecules and form protein-RNA aggregates.
Applications
ChIP, WB
Dilutions
ChIP: 10 µl per ChIP, WB: 1:500 - 1:2,000
Reactivity
Mouse, Human
Immunogen
A peptide derived from within the C-terminus of mouse BRD3.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Mouse BRD3 antibody recognizes bromodomain-containing protein 3, also known as RING3-like protein (RING3L).BRD3 is a member of the bromodomain and extra-terminal motif (BET) protein family. As with other BET proteins, BRD3 associates with acetylated lysine residues on histones and transcription factors (Gamsjaeger et al. 2011) (Lamonica et al. 2011). BRD3 is thought to be involved in nucleosome remodelling in the context of transcription (LeRoy et al. 2008). BRD3 has also been shown to interact with RNA molecules and form protein-RNA aggregates (Daneshvar et al. 2020).
Synonyms
Bromodomain-containing protein 3, KIAA0043, RING3-like protein, RING3L
Western Blot analysis of HeLa whole cell lysate (35 µg; lane 1) and NIH-3T3 whole cell lysate (20 ug; lane 2) probed with Anti-BRD3 Antibody (A320102) at a 1/500 dilution.
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