Recombinant human monoclonal antibody to IL-17RA Receptor.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000
Reactivity
Human
Host
Human
Clonality
Monoclonal
Isotype
IgG2
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human IL-17RA Receptor Protein (6xHis Tag) (A318259) which can bind Anti-IL-17RA Receptor Antibody [Brodalumab Biosimilar] - Azide free (A318918) in a linear range of 3.2-80 µg/ml.
HEK293 cell line transfected with irrelevant protein (B) and human IL17RA (A) were surface stained with Anti-IL-17RA Receptor Antibody [Brodalumab Biosimilar] - Azide free (A318918) at 1 µg/ml followed by Anti-Human IgG Antibody (Alexa 488).
Publishing research using Brodalumab Biosimilar - Anti-IL-17RA Receptor Antibody - BSA and Azide free (A318918)? Please let us know so that we can list the citation on this page.
![ELISA - Anti-IL-17RA Receptor Antibody [Brodalumab Biosimilar] - Azide free (A318918) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318918_1.png?profile=product_top)
![Flow Cytometry - Anti-IL-17RA Receptor Antibody [Brodalumab Biosimilar] - Azide free (A318918) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318918_3.png?profile=product_top)
![ELISA - Anti-IL-17RA Receptor Antibody [Brodalumab Biosimilar] - Azide free (A318918) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318918_1.png?profile=product_top_thumb)
![Flow Cytometry - Anti-IL-17RA Receptor Antibody [Brodalumab Biosimilar] - Azide free (A318918) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318918_3.png?profile=product_top_thumb)
![ELISA - Anti-IL-17RA Receptor Antibody [Brodalumab Biosimilar] - Azide free (A318918) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318918_1.png?profile=product_image)
![Flow Cytometry - Anti-IL-17RA Receptor Antibody [Brodalumab Biosimilar] - Azide free (A318918) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318918_3.png?profile=product_image)