Anti-BTK Antibody [ARC0485] (A308271)

Name: Anti-BTK Antibody [ARC0485]
Brand: Antibodies.com
SKU: A308271
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0485] antibody to BTK for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-BTK Antibody [ARC0485]

Description

Rabbit monoclonal [ARC0485] antibody to BTK.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 560-659 of human BTK (Q06187).

Sequence

PVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0485

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

76 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Agammaglobulinemia tyrosine kinase, AGMX1, ATK, B-cell progenitor kinase, BPK, Bruton tyrosine kinase, Tyrosine-protein kinase BTK

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-BTK Antibody [ARC0485] (A308271)

Western blot analysis of extracts of various cell lines, using Anti-BTK Antibody [ARC0485] (A308271) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-BTK Antibody [ARC0485] (A308271)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-BTK Antibody [ARC0485] (A308271) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-BTK Antibody [ARC0485] (A308271)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-BTK Antibody [ARC0485] (A308271) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-BTK Antibody [ARC0485] (A308271)

Immunofluorescence analysis of mouse spleen using Anti-BTK Antibody [ARC0485] (A308271) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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