Recombinant rabbit monoclonal [DM93] antibody to BTN3A1.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM93
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Anti-BTN3A1 Antibody [DM93] - BSA and Azide free (A318612)
ELISA plates were pre-coated with Recombinant Human BTN3A1 Protein (Fc Chimera 6xHis Tag) (A318356) at 2 µg/ml (100 µl/well) which can bind Anti-BTN3A1 Antibody [DM93] - BSA and Azide free (A318612) in a linear range of 0.64-80 ng/ml.
Flow cytometry analysis of Anti-BTN3A1 Antibody [DM93] - BSA and Azide free (A318612) on Expi293 cells transfected with human BTN3A1 (Red histogram) or Expi293 transfected with irrelevant protein (Blue histogram).
Flow cytometry analysis of antigen binding of Anti-BTN3A1 Antibody [DM93] - BSA and Azide free (A318612). (A) Anti-BTN3A1 Antibody [DM93] - BSA and Azide free (A318612) does not bind to MCF-7 cells that do not express BTN3A1. (B) A clear peak shift of Anti-BTN3A1 Antibody [DM93] - BSA and Azide free (A318612) was seen compared to the control when incubated with BTN3A1-expressing 8226 cells, indicating strong binding of Anti-BTN3A1 Antibody [DM93] - BSA and Azide free (A318612) to BTN3A1. Antibodies were incubated at 2 µg/ml. .
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