Anti-Bu-1a Antibody [L22] (FITC) (A280849)

Anti-Bu-1a Antibody [L22] (FITC)

Mouse monoclonal [L22] antibody to Bu-1a (FITC).

This antibody recognises the 'a' allele of the polymorphic cell surface antigen Bu-1, also known as chB6. Bu-1a is a 70 kDa alloantigen expressed on B cells and a subset of macrophages. Bu-1 is expressed on B cell precursors at early stages of development. Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Mouse anti chicken BU-1a, clone L22 cross reacts with quail Bu-1 but does not appear reactive with either turkey or guinea fowl tissues

Flow Cytometry: 1:20 - 1:80, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Chicken, Quail

This antibody does not cross react with Turkey or Guinea Fowl

Chicken Bursa cells.

Mouse

Monoclonal

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Chicken Bu-1a, clone L22 recognises the 'a' allele of the polymorphic cell surface antigen Bu-1, also known as chB6. Bu-1a is a 70 kDa alloantigen expressed on B cells and a subset of macrophages. Bu-1 is expressed on B cell precursors at early stages of development (Houssaint et al. 1991). Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Mouse anti chicken BU-1a, clone L22 cross reacts with quail Bu-1 but does not appear reactive with either turkey or guinea fowl tissues (Igyarto et al. 2008)

This product is for research use only. It is not intended for diagnostic or therapeutic use.