Recombinant mouse monoclonal [2353] [r2H2] antibody to c-Fos (CF®680R).
Specificity
This antibody recognizes FBJ murine osteosarcoma viral oncogene homolog (FOS), commonly referred to as c-Fos, an immediate-early transcription factor involved in cellular proliferation, differentiation, and stress responses.
Applications
ICC/IF, IHC (Published For Clone ID), WB (Published For Clone ID)
Positive Control
Serum-starved and restimulated HeLa cells
Dilutions
IF: 1-5 µg/mL
Reactivity
Human, Mouse, Rat
Host
Mouse
Clonality
Monoclonal
Clone ID
r2H2
Isotype
IgG1
Light Chains
kappa
Conjugate
CF®680R
Concentration
100 µg/mL
Predicted MW
62 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 2 mg/mLrecombinant BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. CF® Dyes are guaranteed for at least 6 months from data of receipt when stored correctly.
Example immunofluorescence staining of c-Fos in HeLa cells using conjugated anti-c-Fos Antibody [r2H2]. HeLa cells were serum-starved for 48 hours and subsequently stimulated with 20% FBS for 2 hours. Cells were fixed, permeabilized, and stained with 5 µg/ml of anti-c-Fos Antibody [r2H2] (CF®488A). Confocal microscopy revealed strong nuclear c-Fos staining in serum-stimulated cells, consistent with induction of c-Fos expression following serum stimulation.
Publishing research using Recombinant Anti-c-Fos Antibody [r2H2] (CF®680R) (A351984)? Please let us know so that we can list the citation on this page.
Proteins predicted to interact with c-Fos
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Immunofluorescence of anti-c-Fos Antibody [r2H2] (CF®488A) - Anti-c-Fos Antibody [r2H2] (CF®680R) (A351984) -Antibodies.com](https://cdn.antibodies.com/image/catalog/351/A351984_1.png?profile=product_top)
![Immunofluorescence of anti-c-Fos Antibody [r2H2] (CF®488A) - Anti-c-Fos Antibody [r2H2] (CF®680R) (A351984) -Antibodies.com](https://cdn.antibodies.com/image/catalog/351/A351984_1.png?profile=product_top_thumb)
![Immunofluorescence of anti-c-Fos Antibody [r2H2] (CF®488A) - Anti-c-Fos Antibody [r2H2] (CF®680R) (A351984) -Antibodies.com](https://cdn.antibodies.com/image/catalog/351/A351984_1.png?profile=product_image)