Anti-C Reactive Protein Antibody [ARC0341] (A309038)

Western blot analysis of extracts of Human serum, using Anti-C Reactive Protein Antibody [ARC0341] (A309038) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

Western blot analysis of extracts of Mouse serum, using Anti-C Reactive Protein Antibody [ARC0341] (A309038) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-C Reactive Protein Antibody [ARC0341] (A309038) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.