This antibody recognises human C3d and gave a single arc in the alpha region when tested by IEP against aged human plasma (C3d) and a continuous double arc in the alpha and beta regions when tested against fresh human plasma (C3 and C3d). Identity was confirmed by double diffusion (ouchterlony) versus human plasma and a known anti-human C3d.
Applications
IHC-P, ELISA, Radial Immunodiffusion
Dilutions
IHC-P: 1:100
Reactivity
Human
Immunogen
Purified C3d from pooled activated serum.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Ion exchange chromatography.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Glycine Buffered Saline with <0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine, and 1 mM EDTA.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Human C3d antibody (2222-7054) recognizes human C3d and gave a single arc in the alpha region when tested by IEP against aged human plasma (C3d) and a continuous double arc in the alpha and beta regions when tested against fresh human plasma (C3 and C3d). Identity was confirmed by double diffusion (ouchterlony) versus human plasma and a known anti-human C3d.
Flow cytometric analysis ofAnti-C3d Antibody (A282766) used to demonstrate C3d staining in human liver portal triad by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Biotinylated anti sheep IgG followed by alkaline phosphatase conjugated streptavidin (red) were used for detection. Nuclei are counterstained with Anti-C3d Antibody - (A282766).
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