Mouse monoclonal [033D-69.4.1 (10-12)] antibody to C4c.
Specificity
This antibody recognises complement component 4c (C4c). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.
Applications
Flow Cytometry, ELISA, IHC-Fr, WB
Reactivity
Human
Immunogen
Purified human C4c.
Host
Mouse
Clonality
Monoclonal
Clone ID
033D-69.4.1 (10-12)
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of ascites.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Borate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human C4c antibody, clone 033D-69.4.1 (10-12) recognizes complement component 4c (C4c). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.
