This antibody recognizes an extracellular epitope of CD88 protein, a 43 kDa receptor of C5a component of the complement cascade.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Rabbit, Ferret, Bovine, Human
Immunogen
Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR.
Host
Mouse
Clonality
Monoclonal
Clone ID
S5/1
Isotype
IgG2a
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
43 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling C5a-R with Anti-C5a-R Antibody [S5/1] (APC) (A320650), (10 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with C5aR
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.