Immunohistochemistry analysis of mouse brain tissue, fixed in formalin. The Primary Antibody used was Anti-CACNA1C Antibody [S57] (A305124) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Magnification: 40x.
Western Blot - Anti-CACNA1C Antibody [S57] (A305124)
Western blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Anti-CACNA1C Antibody [S57] (A305124) at 1:1,000.
Immunohistochemistry analysis of human hippocampus, fixed in 10% formalin. The Primary Antibody used was Anti-CACNA1C Antibody [S57] (A305124) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-CACNA1C Antibody [S57] (A305124), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X.(A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav1.2 Antibody. (D) Composite.
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-CACNA1C Antibody [S57] (A305124), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.2 Antibody (D) Composite.