Anti-Calcineurin A Antibody (A305119)

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This product is discontinued

Anti-Calcineurin A Antibody (A305119) has been discontinued and is no longer available.

View all Calcineurin A Antibodies.

Specifications

Name

Anti-Calcineurin A Antibody

Description

Rabbit polyclonal antibody to Calcineurin A.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:1,000, ICC/IF: 1:120, IHC: 1:100

Reactivity

Human, Mouse, Rat, Canine, Rabbit

Immunogen

Human Calcineurin A peptide (AA 264-283).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~61 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, CALNA, CAM-PRP catalytic subunit, CNA, CNA alpha, PPP3CA, Protein phosphatase 3 catalytic subunit alpha, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Calcineurin A Antibody (A305119)

Western blot analysis of rat brain cell lysates showing detection of ~61 kDa Calcineurin A protein using Anti-Calcineurin A Antibody (A305119) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~61 kDa.

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Immunofluorescence - Anti-Calcineurin A Antibody (A305119)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Calcineurin A Antibody (A305119), at 1:120 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cell membrane. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Calcineurin A Antibody. (C) Composite.

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Immunohistochemistry - Anti-Calcineurin A Antibody (A305119)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-Calcineurin A Antibody (A305119) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: General epidermis.

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Immunofluorescence - Anti-Calcineurin A Antibody (A305119)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Calcineurin A Antibody (A305119), at 1:120 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cell membrane. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Calcineurin A Antibody. (C) Composite.