Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Calcineurin B homologous protein 1, Calcineurin B-like protein, Calcium-binding protein CHP, Calcium-binding protein p22, CHP, CHP1, EF-hand calcium-binding domain-containing protein p22
Western Blot - Anti-Calcium binding protein P22 Antibody (A88712)
Western blot analysis of extracts of various cell lines, using Anti-Calcium binding protein P22 Antibody (A88712) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-Calcium binding protein P22 Antibody (A88712)
Immunofluorescence analysis of L929 cells using Anti-Calcium binding protein P22 Antibody (A88712) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Calcium binding protein P22 Antibody (A88712)
Immunofluorescence analysis of U-2 OS cells using Anti-Calcium binding protein P22 Antibody (A88712) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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