Calpastatin expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Calpastatin Antibody (A92564) at a dilution of 1:1000 and detected with chemiluminescence.
Calpastatin expression in human stomach tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-Calpastatin Antibody (A92564) at a dilution of 1:100.
Calpastatin expression in human uterine cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-Calpastatin Antibody (A92564) at a dilution of 1:100.
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