Anti-CaMK1-alpha (phospho Thr177) Antibody (A94128)

Name: Anti-CaMK1-alpha (phospho Thr177) Antibody
Brand: Antibodies.com
SKU: A94128
Price: 115.0 GBP
Availability: InStock

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$190

Rabbit polyclonal antibody to CaMK1-alpha (phospho Thr177) for WB, IHC, IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-CaMK1-alpha (phospho Thr177) Antibody

Description

Rabbit polyclonal antibody to CaMK1-alpha (phospho Thr177).

Specificity

This antibody detects endogenous levels of CaMK1-alpha only when phosphorylated at Thr177.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:20000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177 (amino acids 143-192).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

41kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaM-KI, CaMKI-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunofluorescence - Anti-CaMK1-alpha (phospho Thr177) Antibody (A94128)

Immunofluorescence analysis of HeLa cells using Anti-CaMK1-alpha (phospho Thr177) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Immunohistochemistry - Anti-CaMK1-alpha (phospho Thr177) Antibody (A94128)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-CaMK1-alpha (phospho Thr177) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-CaMK1-alpha (phospho Thr177) Antibody (A94128)

Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' and Jurkat cells treated with insulin 0.01U/ml 15' using Anti-CaMK1-alpha (phospho Thr177) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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