The antibody detects endogenous level of CaMK2- beta / gamma / delta only when phosphorylated at Threonine 287.
Applications
ELISA, WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Threonine 287 (Q-E-T(p)-V-E) derived from Human CaMK2- beta / gamma / delta.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from Rat brain using CaMK2- beta/ gamma/ delta (Phospho-Thr287) Antibody #12065.The lane on the right is treated with the antigen-specific peptide.
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