This antibody recognises calcium/calmodulin-dependant protein kinase kinase 2.CaMKK II is part of the serine/threonine kinase family, and is involved in the activation and phosphorylation of targets such as CAMKI, CAMKIV and AMPK. The facilitation of these targets increases the progression of cancer. CamKK II is involved in the regulation of inflammatory conditions such as prostate and hepatocellular cancers, as well as conditions like obesity and insulin resistance.
Applications
WB
Dilutions
WB: 1:2,000
Reactivity
Human
Immunogen
E. coli derived recombinant protein corresponding to amino acids 1-446 of human CaMKK II.
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Molecular Weight
Approximately 75 and 77 kDa in K562 cell lysates.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti human CaMKK II antibody, clone AB01/3H6 recognizes calcium/calmodulin-dependant protein kinase kinase 2.CaMKK II is part of the serine/threonine kinase family, and is involved in the activation and phosphorylation of targets such as CAMKI, CAMKIV and AMPK. The facilitation of these targets increases the progression of cancer (Pulliam et al. 2022). CamKK II is involved in the regulation of inflammatory conditions such as prostate and hepatocellular cancers, as well as conditions like obesity and insulin resistance (Racioppi et al. 2019).
Western Blot - Anti-CAMKK2 Antibody [AB01/3H6] (A320308)
Western Blot analysis of whole cell lysates (25 µg) probed with Anti-CAMKK2 Antibody [AB01/3H6] (A320308) at a 1/2000 dilution followed by detection with Goat anti mouse HRP conjugate at a 1/20000 dilution and visualized on the ChemiDoc MP imaging system. Performed under reduced conditions. Arrows point to CaMKK II (molecular weight 75, 77 kDa).
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