Western Blot - Anti-Carbonic Anhydrase 9/CA9 Antibody (A13577)
Western blot analysis of extracts of various cell lines, using Anti-Carbonic Anhydrase 9/CA9 Antibody (A13577) at 1:100 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
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