Anti-Carbonic Anhydrase 9/CA9 Antibody (A90618)

Western blot analysis of extracts of various cell lines, using Anti-Carbonic Anhydrase 9/CA9 Antibody (A90618) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-Carbonic Anhydrase 9/CA9 Antibody (A90618) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-Carbonic Anhydrase 9/CA9 Antibody (A90618) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using Anti-Carbonic Anhydrase 9/CA9 Antibody (A90618) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).