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Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242)

Western Blot - Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242) - Antibodies.com Immunohistochemistry - Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1275] antibody to Carbonic Anhydrase 9/CA9 for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275]
Description
Rabbit monoclonal [ARC1275] antibody to Carbonic Anhydrase 9/CA9.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Carbonic Anhydrase 9 (CA9/G250) (Q16790).
Sequence
HSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1275
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
35 - 58 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242) - Antibodies.comEnlarge Image

Western Blot - Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242)

Western blot analysis of extracts of various cell lines, using Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-Carbonic Anhydrase 9/CA9 Antibody [ARC1275] (A306242) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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