Anti-Carbonic Anhydrase 9/CA9 Antibody [DM171] - BSA and Azide free
Description
Recombinant rabbit monoclonal [DM171] antibody to Carbonic Anhydrase 9/CA9.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM171
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Flow cytometry analysis of Anti-Carbonic Anhydrase 9/CA9 Antibody [DM171] - BSA and Azide free (A318538) on Expi293 cells transfected with human CA9 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of Anti-Carbonic Anhydrase 9/CA9 Antibody [DM171] - BSA and Azide free (A318538). (A) Anti-Carbonic Anhydrase 9/CA9 Antibody [DM171] - BSA and Azide free (A318538) does not bind to Jurkat cells that do not express CA9. (B) A clear peak shift of Anti-Carbonic Anhydrase 9/CA9 Antibody [DM171] - BSA and Azide free (A318538) was seen compared to the control when incubated with CA9-expressing 8226 cells, indicating strong binding of Anti-Carbonic Anhydrase 9/CA9 Antibody [DM171] - BSA and Azide free (A318538) to CA9. Antibodies were incubated at 5 µg/ml. .
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![Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 Antibody [DM171] - BSA and Azide free (A318538) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318538_1.jpg?profile=product_image)
![Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 Antibody [DM171] - BSA and Azide free (A318538) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318538_2.jpg?profile=product_image)