Anti-Caspase-3 Antibody [ARC0143] (A308685)

Western blot analysis of extracts of various cell lines, using Anti-Caspase-3 Antibody [ARC0143] (A308685) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Caspase-3 Antibody [ARC0143] (A308685) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Caspase-3 Antibody [ARC0143] (A308685) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using Anti-Caspase-3 Antibody [ARC0143] (A308685). HeLa cells were treated by Etoposide (25 uM) at 37°C for 5 hours. DAPI was used to stain the cell nuclei (blue).