Anti-Caspase-5 Antibody (A283987)

Anti-Caspase-5 Antibody

Rabbit polyclonal antibody to Caspase-5.

This antibody recognises an epitope located within the internal region of human Caspase-5, a ~47 kDa cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma. In vitro cleavage of caspase-5 by the serine protease granzyme B, which plays a major role in cytotoxic T lymphocyte (CTL)-induced apoptosis.

WB: 1 µg/ml - 2 µg/ml

Human

A peptide corresponding to 14 amino acids from the centre of human Caspase-5.

Rabbit

Polyclonal

IgG

Affinity chromatography.

Approximately 48 kDa in Ramos cell lysates. (Predicted precursor MWT 47.8 kDa)

Liquid

Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human Caspase-5 antibody recognizes an epitope located within the internal region of human Caspase-5, a ~47 kDa cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes.Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta.The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma. In vitro cleavage of caspase-5 by the serine protease granzyme B, which plays a major role in cytotoxic T lymphocyte (CTL)-induced apoptosis (Wang et al. 1996).

CASP-5, CASP5, ICE(rel)-III, ICH3, Protease ICH-3, Protease TY