Anti-Caspase 8 (cleaved Asp384) Antibody (A95120)

Name: Anti-Caspase 8 (cleaved Asp384) Antibody
Brand: Antibodies.com
SKU: A95120
Price: 115.0 GBP
Availability: InStock

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This product is discontinued

Anti-Caspase 8 (cleaved Asp384) Antibody (A95120) has been discontinued and is no longer available.

View all Caspase-8 Antibodies.

Specifications

Name

Anti-Caspase 8 (cleaved Asp384) Antibody

Description

Rabbit polyclonal antibody to Caspase 8 (cleaved Asp384).

Specificity

This antibody detects endogenous levels of fragment of activated Caspase 8 resulting from cleavage adjacent to Asp384.

Applications

WB, ELISA

Dilutions

WB: 1:500~1:1000, ELISA: 1:10000

Reactivity

Human

Immunogen

Synthetic peptide derived from human Caspase 8 (amino acids 335-384).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

47 and 55kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, MCH5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-Caspase 8 (cleaved Asp384) Antibody (A95120)

Western blot analysis of lysates from 293 cells, treated with etoposide 25uM 1h using Anti-Caspase 8 (cleaved Asp384) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Western Blot - Anti-Caspase 8 (cleaved Asp384) Antibody (A95120)

Western blot analysis of various cells using Anti-Caspase 8 (cleaved Asp384) Antibody.

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Immunohistochemistry - Anti-Caspase 8 (cleaved Asp384) Antibody (A95120)

Immunohistochemical analysis of paraffin-embedded human breast tissue using Anti-Caspase 8 (cleaved Asp384) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.

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Immunohistochemistry - Anti-Caspase 8 (cleaved Asp384) Antibody (A95120)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Anti-Caspase 8 (cleaved Asp384) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.