Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BR22, Coiled-coil domain-containing protein 59, TAP26, Thyroid transcription factor 1-associated protein 26, TTF-1-associated protein 26, TTF-1-associated protein BR2
Western blot analysis of extracts of various cell lines, using Anti-CCDC59 Antibody (A89105) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-CCDC59 Antibody (A89105) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-CCDC59 Antibody (A89105) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Anti-CCDC59 Antibody (A89105) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
