This antibody targets the C-terminal region of human CCKAR, recognizing the non-phosphorylated isoform. It is optimized for Western blot analysis, facilitates immunoprecipitation from cell and tissue lysates, and is suitable for immunohistochemical staining in cultured cells and tissue sections.
Applications
WB, ICC, IHC
Dilutions
WB: 1:1,000, ICC: 1:200, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to human CCKAR (amino acids. 409-428). Immunogen range is 22-22 amino acids.
Sequence
TGASLSRFSYSHMSASVPPQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CCK-A receptor, CCK-AR, CCK1-R, CCKRA, Cholecystokinin receptor type A, Cholecystokinin-1 receptor
Western blot validation of Cholecystokinin Receptor 1 (CCKAR) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing CCKAR were immunoblotted with anti-CCKAR antibody (A334502) at a 1:1000 dilution.
Immunocytochemical detection of Cholecystokinin Receptor 1 (CCKAR) in HEK293 cells. HEK293 cells stably expressing CCKAR were untreated or treated with 1 µM CCK-8 for 30 min and stained with anti-CCKAR antibody (A334502) at 1:200. CCKAR receptors were observed at the plasma membrane in untreated cells (0 min) and in perinuclear vesicular clusters after 30 min of CCK-8 exposure.
Immunohistochemical localization of Cholecystokinin Receptor 1 (CCKAR) in gastric mucosa. Gastric mucosa sections were dewaxed, microwaved in citric acid, and incubated with anti-CCKAR antibody (A334502) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin. CCKAR receptors were uniformly detected at the plasma membrane of chief cells.
Detection of Cholecystokinin Receptor 1 (CCKAR) in neuroendocrine tumor by immunohistochemistry. Tumor sections were dewaxed, microwaved in citric acid, and stained with anti-CCKAR antibody (7A334502) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were developed with 3,3-diaminobenzidine (DAB)-glucose oxidase and counterstained with hematoxylin. CCKAR receptors were predominantly observed at the plasma membrane of tumor cells.
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