This antibody recognises human IGFBP-9 (insulin-like growth factor binding protein- 9), also known as NOV/CCN3, a 326 amino acid cysteine-rich secreted regulatory protein and member of the CCN family, containing the domains IGFBP, VWFC, C-terminal cysteine knot-like and thrombospondin type I repeat.IGFBP-9 is an inducer of cell migration and adhesion and shows increased expression in Wilm’s tumours and nephroblastomas. In contrast, decreased expression of IGFBP-9 correlates with an increase in tumourgenicity in certain cancers including childhood adrenocortical tumors (ACT).
Applications
WB, ELISA, IHC-P
Dilutions
ELISA: 0.5 - 2 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivity
Human
Immunogen
Recombinant human IGFBP-9.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
1 mg/ml after reconstitution.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti Human IGFBP-9 antibody recognizes human IGFBP-9 (insulin-like growth factor binding protein- 9), also known as NOV/CCN3, a 326 amino acid cysteine-rich secreted regulatory protein and member of the CCN family, containing the domains IGFBP, VWFC, C-Terminal cysteine knot-like and thrombospondin type I repeat.IGFBP-9 is an inducer of cell migration and adhesion and shows increased expression in Wilm’s tumours and nephroblastomas. In contrast, decreased expression of IGFBP-9 correlates with an increase in tumourgenicity in certain cancers including childhood adrenocortical tumors (ACT).
Synonyms
CCN family member 3, Cellular communication network factor 3, IBP-9, IGF-binding protein 9, IGFBP-9, IGFBP9, Insulin-like growth factor-binding protein 9, Nephro blastoma-overexpressed gene protein homolog, NOV, NovH, Protein NOV homolog
